AutoPrep Automated Chromatography System is applicable to the pilot scale and large-scale production of bio-pharmaceutical purification processes such as monoclonal antibody, insulin, blood products, vaccine and recombinant protein. As an integrated control unit, it can automatically realize the column equilibration, sample loading, elution, collection, regeneration and cleaning in the purification and separation process with compliance of GMP guideline. The system can provide a variety of flexible configurations to meet the needs of large-scale production.